This article focuses on the bit sign transition and its impact on the acquisition performance in terms of probability of detection. To do so, a theoretical study on the correlation process considering bit sign transition is done leading to the expression of the probability of detection, expressed in function of the bit transition location. Based on this, Monte-Carlo simulations were run to determine the acquisition performance degradations in terms of sensitivity losses and probability of detection for several GNSS civil signals. This allows determining the optimal acquisition parameters when bit sign transitions are considered during the acquisition process.